Isolation and Identification of Antifungal Compounds Produced by Bacillus Y-IVI for Suppressing Fusarium Wilt of Muskmelon

Zhao Q., Mei X., Xu Y. (2016): Isolation and identification of antifungal compounds produced by Bacillus Y-IVI for suppressing Fusarium wilt of muskmelon. Plant Protect. Sci., 52: 167–175. The Bacillus strain Y-IVI was used in the biocontrol of muskmelon Fusarium wilt. It was identified as Bacillus subtilis . The antifungal compounds from the culture filtrate were purified by high performance liquid chromatography. Two series of homologous ion peaks were analysed by liquid chromatography-electrospray ionisation-mass spectrometry, one with molecular weights of 1028.7, 1042.7, and 1056.7 and the other with molecular weights of 1463, 1477, and 1491. The compounds were ascribed to iturin A and fengycin, respectively. The maximum production of iturin by Y-IVI was 89.75 mg/l. In conclusion, we provided biochemical evidence that strain Y-IVI was able to produce antifungal compounds and thus holds great potential for use in the biocontrol of Fusarium wilt disease.